The Polymerase Chain Reaction (PCR) for Human Viral DiagnosisThe polymerase chain reaction (PCR) is the most sensitive method for revealing the presence of otherwise undetectable quantities of the genome of RNA or DNA of human viruses. The Polymerase Chain Reaction (PCR) for Human Viral Diagnosis addresses the urgent need to use this revolutionary technology in reference and routine diagnostic laboratories. It informs the molecular biologist of the most appropriate clinical uses for PCR and educates the clinician and medical virologist about the subtleties and benefits of gene amplification. The reader is given an understanding and appreciation of the principles of PCR and how, why, and where it should be applied. The book explains the principles behind PCR and its role in the diagnostic and public health laboratory. The application of PCR to the detection and investigation of viral latency and persistence is presented by the originators of in situ amplification. There are individual contributions from experts in their respective fields on the detection, characterization, and analysis by PCR of gastroenteritis viruses, hepatitis viruses, herpesviruses, rhinoviruses, enteroviruses, flaviviruses, polyomaviruses, human immunodeficiency virus (HIV), human T-lymphotropic virus types I and II (HTLV-I and II); and measles, mumps, rubella, influenza, rabies, and B19 viruses. |
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Acids Res acute amplicons analysis antibody antigen B19 DNA cDNA characterization chronic Clin clinical cloning containing cytomegalovirus dengue diagnosis disease encephalitis enteroviruses enzyme epidemiology Epstein-Barr virus extraction flavivirus gastroenteritis gene genetic genome genotypes hepatitis B virus hepatitis C virus hepatitis E herpes simplex HTLV-I and HTLV-II HTLV-I/II HTLV-II infection human immunodeficiency virus hybridization identified influenza laboratory Lancet measles Meth method Microbiol molecular mumps nested PCR Nucl nucleic acid nucleotide nucleotide sequence oligonucleotide patients PBMCs PCR amplification PCR assays PCR product picornaviruses polymerase chain reaction primer pairs probe protein rabies region reverse transcription rotavirus rotaviruses rubella samples sensitivity sero-genotype serological serotypes serum Southern blot specific specimens SRSV strains studies target techniques tissue transmission vaccine viral DNA Virol Virology virus DNA virus genome virus infection virus isolation virus type viruses